Tramtrack protein-DNA interactions. A cross-linking study.

نویسندگان

  • D E Kamashev
  • A V Balandina
  • V L Karpov
چکیده

Interaction of the Tramtrack protein from Drosophila melanogaster with DNA was analyzed by a cross-linking method. Tramtrack residues cross-linkable to the partially depurinated DNA were identified by direct sequencing. The N-terminal alpha-amino group of the protein DNA-binding domain was found to be the major product of cross-linking. The location of the N terminus on the DNA was determined by identification of the DNA bases that were cross-linked to the protein alpha-amino group. We conclude that accessory N-terminal peptide preceding the first zinc finger of Tramtrack directly interacts with DNA, both in specific and nonspecific DNA-protein complexes. Our finding explains the role in the protein binding of the DNA bases outside of the direct interaction with the zinc fingers.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 275 46  شماره 

صفحات  -

تاریخ انتشار 2000